Akademik Veri Yönetim Sistemi
International Journal of Biological Macromolecules, cilt.316, 2025 (SCI-Expanded)
This study explores a novel approach for the treatment of colorectal cancer (CRC) by evaluating the therapeutic effects of oxaliplatin (OX) and Moringa oleifera (MO) through the use of 3D printed implantable patches composed of polycaprolactone and gelatin polymers. This represents the first use of 3D printed PCL/GE patches for MO delivery in CRC, offering a promising strategy for localized therapy. Traditional CRC treatments, including surgery and chemotherapy, often result in suboptimal outcomes and severe side effects, while cancer cells increasingly show resistance to conventional treatments. We developed implantable patches using PCL/GE based polymers loaded with either OX or MO at various concentrations (50–500 μg/mL). In vitro studies using HCT 116 colon cancer cells showed that MO loaded patches demonstrated superior antiproliferative effects compared to OX loaded patches. The patches exhibited enhanced drug release in acidic conditions (pH 4), simulating the tumor microenvironment. Notably, while OX loaded patches induced necrotic cell death, MO loaded patches triggered controlled apoptotic cell death without inflammation, as evidenced by increased LDH activity and reduced expression of apoptotic genes. This research presents an innovative treatment strategy, providing controlled drug release, reduced systemic toxicity compared to systemic chemotherapy, and improved drug delivery, potentially overcoming conventional chemotherapy limitations.