Cleavage effect of oligoribonucleotides substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides


Hosono K., Gozu H. , Hosaka H., Sakamoto K., Yokoyama S., Takai K., et al.

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, cilt.1354, ss.211-218, 1997 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 1354 Konu: 3
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1016/s0167-4781(97)00099-7
  • Dergi Adı: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
  • Sayfa Sayısı: ss.211-218

Özet

The precursor of an RNA molecule from T4-infected E. coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions [UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. In order to determine the influence of the pyrimidine and purine bases on these sequence-specific cleavage reactions, we studied the cleavage reactions of hairpin loop RNAs substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides. The cleavage was affected by the 2'-hydroxyl groups and the bases of the pyrimidines, and the 6-amino group of the purine. (C) 1997 Elsevier Science B.V.