Akademik Veri Yönetim Sistemi
International Conference on Modern Research in Biological, Pharmaceutical, Medical and Environmental Sciences (ICMRBPMES-24), Frankfurt, Almanya, 16 Aralık 2024, ss.8, (Özet Bildiri)
The genus Sideritis, belonging to the Lamiaceae family, is
represented in Türkiye by 46 species, 36 of which are endemic. The endemic Sideritis trojana Bornm., commonly
known as "Kazdağı tea," was collected from Çanakkale. The aerial
parts of S. trojana were extracted using n-hexane (STH), chloroform
(STK), methanol fractionally (STM1), direct methanol (STM2), and distilled
water (STW) through the percolation method. These five extracts were evaluated for
their anticholinergic, antimicrobial, and antioxidant activities. Additionally,
the total phenolic and flavonoid contents of the extracts were quantified. The
results indicated that the total phenolic content of the extracts ranged from
15.63 to 33.93 mg GAE/g extract, while the flavonoid content ranged from 23.04
to 32.60 mg CE/g extract. The IC50 values (mg/mL) of the extracts
were determined through various antioxidant assays (DPPH, ABTS, FRAP, and superoxide)
and compared to rutin. The most effective extracts in all antioxidant assays were
STM1 and STM2. Moreover, STM1 and STM2 extracts inhibited AChE and BuChE
enzymes in a dose-dependent manner. At 1.25 mg/mL, the AChE and BuChE
inhibition activities of STM1 (IC50: 80.55 ± 0.880 mg/mL, 74.29 ±
1.930 mg/mL) and STM2 (IC50: 80.04 ± 1.670 mg/mL, 79.02 ± 0.430
mg/mL) were found to be comparable to those of galantamine (IC50: 97.62 ± 0.480
mg/mL, 94.25 ± 0.150 mg/mL) at 0.1 mg/mL. None of the extracts exhibited antimicrobial
activity (MIC) against the Gram-positive and Gram-negative bacterial strains
tested. However, the STH extract showed activity against Candida albicans,
with a MIC value of 325 mg/mL.
Keywords: S.
trojana, antioxidant, anticholinergic, antimicrobial
Acknowledgement: This study was supported by TUBITAK 2209-A (project number:
1919B012103016).