Use of KASP Assay in Sex Determination of Pistacia


Şahin Z. N. , Aydın Y., Onay A., Altınkut Uncuoğlu A.

International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 26 - 27 Nisan 2018, sa.1465, ss.47

  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.47

Özet

The genus Pistacia is a perennial plant and belongs to Anacardiaceae (Cashew) family. The fruits of

species have an extreme importance in food, health and baking industry as a raw material. While the

species have different characters like membranous, leather, deciduous or evergreen, the most common

known character is dioecism of individuals. The dioecious character (being a male/female) affects the

anatomical or structural traits of individuals like picking season or fruit size so identify the individual is

very crucial for producers. Marker Assisted Selection (MAS) is a promising process and may help to

screen the sex or many other traits of plant seedlings quickly before they reach reproduction stage or show

morphological data in more than several years. In this research, eight different Pistacia species were

analysed with Competitive Allele Specific PCR (KASP) assay as a marker screening system and it was

focused on possible gender determination based on single nucleotide polymorphism (SNP). On the result

of KASP assay, 167992 (A/T) SNP discriminated the three female individuals of Pistacia which P.

atlantica Desf, P. vera L. and P. terebinthus L., 133396 (C/G) SNP marker discriminated only P. vera L.

female. This result was supportive to Pistacia’s ZW/ZZ (female/male) sex determination system because

all discriminated females show heterozygous character as reported. This research has been supported by

Marmara University Research Foundation (Project No: FEN-C-YLP-090217-0049).