International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 26 - 27 Nisan 2018, sa.1465, ss.47
The genus Pistacia is a perennial plant and belongs to Anacardiaceae (Cashew) family. The fruits of
species have an extreme importance in food, health and baking industry as a raw material. While the
species have different characters like membranous, leather, deciduous or evergreen, the most common
known character is dioecism of individuals. The dioecious character (being a male/female) affects the
anatomical or structural traits of individuals like picking season or fruit size so identify the individual is
very crucial for producers. Marker Assisted Selection (MAS) is a promising process and may help to
screen the sex or many other traits of plant seedlings quickly before they reach reproduction stage or show
morphological data in more than several years. In this research, eight different Pistacia species were
analysed with Competitive Allele Specific PCR (KASP) assay as a marker screening system and it was
focused on possible gender determination based on single nucleotide polymorphism (SNP). On the result
of KASP assay, 167992 (A/T) SNP discriminated the three female individuals of Pistacia which P.
atlantica Desf, P. vera L. and P. terebinthus L., 133396 (C/G) SNP marker discriminated only P. vera L.
female. This result was supportive to Pistacia’s ZW/ZZ (female/male) sex determination system because
all discriminated females show heterozygous character as reported. This research has been supported by
Marmara University Research Foundation (Project No: FEN-C-YLP-090217-0049).