Effects of gallic acid on expressions of MMP-2 and MMP-9 through the pathway of p38/JNK in C6 glioma cells

Yaka E. B., Yazici Z. M. C., Ersoz M., GÜLSOY N.

PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.36, sa.1, ss.59-66, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.36721/pjps.2023.36.1.reg.059-066.1
  • Dergi Adı: PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.59-66
  • Anahtar Kelimeler: Matrix metalloproteinase, oxidative damage, gallic acid, glioma, toxicity, CANCER-CELLS, JNK, PROLIFERATION, INVASION, PROTEIN, BREAST, ANGIOGENESIS, INHIBITOR, VIABILITY, SP600125
  • Marmara Üniversitesi Adresli: Evet

Özet

In our study, the effects of gallic acid (GA), a natural therapeutic agent, on oxidative stress biomarkers and MMP-2 and MMP-9 expressions via the p38/JNK pathway in C6 glioma cells were investigated. The toxicity of GA was determined by the WST-1 method. JNK, p38 and MMP-2/-9 mRNA expressions in the cell line were detected by RT-qPCR. JNK/SAPK, Grap-2/p38 and MMP-2/-9 protein levels were analyzed by using ELISA methods. Biochemical markers were analyzed. GA reduced the cell viability of C6 glioma cells after 24, 48 and 72h of treatment. The expression levels of MMP-2 and MMP-9 mRNA decreased in C6 glioma cells treated with 150 mu g/ml GA for 24 and 48h compared to the control cells. Unlike SOD activity, GA treatment significantly increased PCO and MDA levels in the cells treated with 150 mu g/ml GA for 24 and 48h compared to the non-treated cells. According to our results, GA inhibited the proliferation of C6 glioma cells. Also, it reduced MMP-2 and MMP-9 expressions and increase oxidative stress. Therefore, GA may have preventive effects on gliomas progression and/or invasion.