This study aims to investigate the relationships between organizational cultures, spirit at work, and organization based self-esteem among academicians, and whether organization based self-esteem mediates the relationship between organizational culture and spirit at work. A cross-sectional design was employed, and data were collected through a questionnaire-based survey. Based on the convenience sampling, 417 academicians answered the questionnaire in public and private universities located in Turkey. To analyze the data descriptive statistics, Pearson coefficient, simple and multiple regression analyses were conducted. Feminine organizational culture has more positive contribution on experiencing spirit at work than masculine organizational culture. Moreover, organization based self-esteem does not mediate the relationship between types of organizational culture and spirit at work. Results suggest that organizations hoping to enhance their employee's spirit at work could focalize creating an organizational environment that fosters employee spirit at work. The originality of this paper is that it creates awareness and emphasizes potential contributors that foster an individual. s experience of spirit at work.