Lipoprotein lipase gene polymorphism and lipid profile in coronary artery disease


Duman B., Türkoǧlu Ç., Akpinar B., Güden M., Vertii A., Dak E., ...Daha Fazla

Archives of Pathology and Laboratory Medicine, cilt.128, sa.8, ss.869-874, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 128 Sayı: 8
  • Basım Tarihi: 2004
  • Dergi Adı: Archives of Pathology and Laboratory Medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.869-874
  • Marmara Üniversitesi Adresli: Hayır

Özet

Context.-Lipoprotein lipase (LPL) plays a central role in lipid metabolism, hydrolyzing triglyceride in chylomicrons and very-low-density lipoproteins. The Pvull polymorphic variant of LPL gene is common and might affect risk of coronary artery disease (CAD). Objective.-Our aim was to determine whether LPL-Pvull polymorphism can be considered to be an independent risk factor or a predictor for CAD in Turkish subjects. Design.-We used polymerase chain reaction and restriction enzyme digestion to determine the distribution of the previously described C→T transition that causes a Pvull polymorphism in intron 6 among healthy blood donors of Turkish origin and among angiographically confirmed CAD patients with comparable ethnic backgrounds. Results.-For the Pvull genotypes, within the CAD group (n = 80), the +/- genotype was found in 39 individuals (48.8%), whereas 25 (31.3%) carried the +/+ genotype, and 14 (17.5%) carried the -/- genotype. Within the control group (n = 49), the -/- genotype was found in 19 individuals (38.8%), 16 (32.7%) carried the +/- genotype, and 14 (28.6%) carried the +/+ genotype. The genotype frequency distribution was significantly different (P = .049) in the CAD and control study groups. The most frequent genotype among CAD patients was +/-; this genotype was more frequent in patients than in control subjects. However, the -/- genotype was more prevalent in the control group. Lipoprotein lipase-Pvull polymorphism was found to be associated with fasting total cholesterol and low-density lipoprotein cholesterol levels. The +/+ genotype was found to have higher levels of total cholesterol and low-density lipoprotein cholesterol in both the CAD and control groups. Conclusion.-There was a difference in the distribution of LPL-Pvull genotypes between the healthy subjects and the patients with CAD. Lipoprotein lipase-Pvull polymorphisms were not detected as independent risk factors for CAD in this study group, but had associations with lipid levels.