Use of coloured lighting in interior spaces has become prevalent in recent years. Considerable importance is ascribed to coloured lighting in interior and lighting design. The effects of colour on the perception of interior spaces have been studied as surface colour; but here, the effects of three different types of chromatic light were investigated. The lighting differed in colour (red, green and white) and perceptions of interior space were assessed. 97 participants (59 women, 38 men; M age = 21.4 yr.) evaluated the experiment room on a questionnaire assessing eight evaluative factors: Pleasantness, Arousal, Aesthetics, Usefulness, Comfort, Spaciousness, Colour, and Lighting quality. Perceptions of the room differed by colour of lighting for some of the evaluative factors, but there was no sex difference in perceptions. Interior spaces may be perceived as equally pleasant under white, green and red lighting. Under white lighting a space is perceived as more useful, spacious, clear, and luminous. Green lighting would make the same effect. Green and white lighting were perceived equally comfortable in an interior space. Chromatic coloured lighting was perceived to be more aesthetic than white lighting. The results support previous findings for some evaluative factors, but differed for others.