Fresenius Environmental Bulletin, cilt.29, sa.12, ss.11175-11179, 2020 (SCI Expanded İndekslerine Giren Dergi)
Tribenuron-methyl (TBM) is a sulfonylurea group herbicide commonly used in weed control and integrated pest control in the world and in our coun-try. As with all pesticides, its incorporation into the aquatic environment is dangerous for non-target or-ganisms. Zebrafish (Danio rerio) is a freshwater fish that has been used frequently as a bioindicator spe-cies in the field of environmental toxicology in re-cent years. In this study, zebrafish were fed with pel-let feed in tanks filled with chlorine-free water for 10 fish in each aquarium for two weeks. Experimental groups were exposed to 25, 50 and 100 ppm doses of TBM for 96 hours. The experiments were performed in triplicate. At the end of the period the liver organs of the fish were dissected and homogenized immedi-ately. Then, reduced glutathione (GSH), malondial-dehyde (MDA), total protein (TP) levels and acetyl-cholinesterase (AChE) and catalase (CAT) enzyme activities were determined by spectrophotometric methods.
According to the findings, TP level and CAT enzyme activity increased in all doses compared to the control group. MDA levels decreased at all doses. AChE enzyme activity decreased at 25 and 50 ppm doses and increased at 100 ppm dose group. GSH levels increased in the 25 and 100 ppm group, but decreased in the 50 ppm group. In conclusion, TBM showed a detrimental effect on liver tissue en-zyme activities and lipid peroxidation levels of zebrafish.