Characterization of the secretome of human tooth germ stem cells (hTGSCs) reveals neuro-protection by fine-tuning micro-environment

Yalvac M. E., YARAT A., Mercan D., Rizvanov A. A., Palotas A., ŞAHİN F.

BRAIN BEHAVIOR AND IMMUNITY, cilt.32, ss.122-130, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.bbi.2013.03.007
  • Dergi Adı: BRAIN BEHAVIOR AND IMMUNITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.122-130
  • Anahtar Kelimeler: Bio-active molecules, Cytokine, Human tooth germ stem cells (hTGSCs), Neuro-protection, Secretome, Toxicity, MESENCHYMAL STROMAL CELLS, GROWTH-FACTOR, BONE-MARROW, IN-VITRO, DIFFERENTIATION, TRANSPLANTATION, MIGRATION, SCLEROSIS, INJURY, TISSUE
  • Marmara Üniversitesi Adresli: Evet

Özet

Bone-marrow-derived mesenchymal stem cells (MSCs) demonstrate neuro-protective effects in several disease models. By producing growth-factors, cytokines and chemokines, they promote survival of neurons in damaged brain areas. Alternative MSC sources, such as human tooth germ stem cells (hTGSCs), have been investigated for their neuro-protective properties. They ameliorate effects of neuro-toxic agents by paracrine mechanisms, however these secreted bin-active molecules are not yet characterized. Therefore, the current study aimed to provide a detailed analysis of the secretome of hTGSCs. Brain cells were exposed to various toxic materials, including Alzheimer's beta-amyloid peptide (beta-AP) and 6-hydroxy-dopamine (6-OHDA). When co-cultured with hTGSCs, the activity of a number of anti-oxidant enzymes (catalase, glutathione-s-transferase, glutathione-peroxidase, superoxide-dismutase) was increased and neuronal death/apoptosis was subsequently reduced. The composition of the secreted bio-active materials is influenced by various pre-existing factors such as oxygen and glucose deprivation and the age of cells (passage number). This report reveals for the first time that the neuro-protective secretome of hTGSCs and the micro-environment of cells have a mutual and dynamic impact on one another. (C) 2013 Elsevier Inc. All rights reserved.