A composite nanofiber composed of three polymers, namely polyvinyl alcohol/polyvinyl pyrrolidone/polyethylene oxide, is produced. The experiments are constructed using three design of experiment techniques, Taguchi L-9, Taguchi L-27, and Screening method. The experiments are verified using the analysis of variance (ANOVA) method and later a mathematical model is developed using the regression method. The impact of electrospun processing parameters, namely applied voltage, flow rate, and working distance, on nanofibers' diameter is measured. The working distance is a significant factor in controlling the size of the fiber diameter, while the applied voltage has the lowest effect on it. As a result of the regression equation, a Genetic algorithm is used to find the optimum variables for the required fiber diameter, which is 156 nm for flow rate = 0.001 mL h(-1), voltage = 30 kV, and distance = 200 mm with a 3% difference from the experimental fiber diameter.