Biosynthesis of nano-hydroxyapatite from potential probiotic Bacillus amyloliquefaciens and its effect on osteoblastic cells

Nouri S., Emtiazi G., Roghanian R., ULAĞ S., GÜNDÜZ O.

Materials Letters, cilt.392, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 392
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.matlet.2025.138549
  • Dergi Adı: Materials Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Biomaterials, Bone, Hydroxyapatite, Nanoparticles, Probiotics
  • Marmara Üniversitesi Adresli: Evet

Özet

The eco-friendly synthesis of nano-biomaterials has gained prominence in bone disorder treatment. This study explores the green synthesis of nano-hydroxyapatite using Bacillus amyloliquefaciens and evaluates its biocompatibility and osteogenic potential in MG-63 and MC3T3-E1 cell lines. Characterization confirmed desirable crystallinity and elemental composition. The nanoparticles exhibited no significant toxicity over 21 days, enhancing matrix mineralization and alkaline phosphatase activity (0.44 U/mg in MG-63, 0.55 U/mg in MC3T3-E1). These findings highlight the potential of probiotic-derived nano-Hydroxyapatite for bone tissue engineering and regenerative medicine.