In vitro NMR proton T-1 measurements in peritoneal ascites


YILMAZ A., TEZ M. , GÖRAL V., Boylu S., Kaplan A., Kavak G.

PHYSICS IN MEDICINE AND BIOLOGY, cilt.41, ss.539-549, 1996 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 41 Konu: 3
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1088/0031-9155/41/3/014
  • Dergi Adı: PHYSICS IN MEDICINE AND BIOLOGY
  • Sayfa Sayıları: ss.539-549

Özet

The proton spin-lattice relaxation rate (1/T-1) in malignant and non-malignant ascites was measured with an FT NMR spectrometer operating at 60 MHz. The mean relaxation rate in non-malignant ascites was significantly smaller than that of malignant ascites. However, the 1/T-1 of malignant ascites overlaps with that of non-malignant ascites over all concentrations of total protein (TP) in samples. The 1/T-1 in non-malignant ascites correlates strongly with TP, whilst the 1/T-1 in malignant ascites shows only a moderate correlation. T-1 measurements before and after addition of ascorbic acid (reductant) suggest that there is a small paramagnetic contribution of ions to the 1/T-1 in malignant ascites. The least-squares fitting of 1/T-1 versus TP for non-malignant data gives a linear relationship, and suggests that the T-1 mechanism in non-malignant ascites is caused by a fast chemical exchange of water molecules between protein-bound water and free water.