13th International Conference on Protein Stabilization / ProtStab2021 , Plovdiv, Bulgaristan, 7 - 09 Ekim 2021, ss.34
Nowadays, we are facing
with increasing environmental issues. Replacement of chemical routes with
biotechnological routes which use renewable resources for the production of
value-added biochemicals is not only sustainable but also crucial to reduce
carbon emissions and waste. Extremophilic microorganisms are valuable
biocatalysts using different wastes for the production of industrially
important biochemicals, such as enzymes and biopolymers (1–3). Crustacean
wastes of seafood markets and restaurants have an enormous volume. Shrimp waste
is one of those wastes of which annual production reaches up to 6-8 million
tons in India and countries of the Far East (4). This amount of
waste can be evaluated as a valuable resource for the production of enzymes. In
the light of this information, our aim in this study is to evaluate shrimp
waste as a nitrogen source for the production of alkaliphilic protease.
Bacillus marmarensis is used as the biocatalyst for the valorization of shrimp
waste to produce protease. Productions were conducted in duplicate and protease
activities were conducted spectrophotometrically as mentioned in literature (5). Firstly,
different carbon sources supplementing basal medium were tested to find the
highest protease activity. Then nitrogen source is replaced with different
concentrations of shrimp waste to find the optimum condition with the highest
protease activity. Our results showed that sucrose is the optimum carbon source
and 3% is the optimum shrimp waste concentration.
Our results are promising
in terms of circular economy perspective. Not just shrimp, but also other
agro-industrial wastes are cheap for sustainable production of valuable
biochemicals.