This study was performed in ticks collected with the flagging method from localities situated along Anatolian side of Istanbul to the Bulgarian border of Turkey which is under the effect of Black Sea climate. All ticks which were collected seasonally were screened for the presence of B. burgdorferi s.l. and Rickettsia spp. in pools. As a result, indicated agents were revealed to be common in ticks of studied localities, lxodes ricinus being the predominant tick which was active throughout the year.