Akademik Veri Yönetim Sistemi
1st International Symposium of Biodiversity Studies, Ankara, Türkiye, 23 Mayıs 2022, ss.84
The genus Melissa is used as antibacterial, sedative, digestive aid, carminative, diaphoretic and bile secretion enhancer. It is included in the composition of the preparations used during nervous digestive system disorders, psychosomatic heart disorders and migraine. The flowering branches and leaves of Melissa officinalis L. subsp. altissima. are used as carminative, sedative and its antipyretic effects. It is also used in vascular occlusion, stomach aches, heart ailments, kidney inflammation, cholesterol, blood pressure, and diabetes. According to our knowledge there are not enough studies on these species grown in Turkey. The aim of this study is to examine the antioxidant, cytotoxic, anticholinesterase, anti-urease and antimicrobial activities of plant’ various extracts obtained by using the sequential maceration method. In the determination of antimicrobial activity well diffusion method on 6 gram negative, 4 gram positive and 3 yeast strains was used. DPPH, CUPRAC and FRAP techniques were used to examine the antioxidant properties of plant' extracts. The extracts' anticholinesterase, cytotoxic and anti-urease activity were determined using the Ellman, MTT and Indophenol techniques, respectively. In addition, the phenolic contents of the extracts were determined by FCR method. It was found that petroleum ether extract of the plant was effective against Enterecoccus faecalis and Staphylococcus aureus strains and ethyl acetate extract were effective against Staphylococcus aureus strains. The methanol extract of the plant exhibited the highest antioxidant activity when compared to other extracts. It was determined that the petroleum ether extract of Melissa officinalis L. subsp. altissima has stronger acetylcholinesterase and urease enzyme inhibition potential than its other extracts. Furthermore, in this study the ethyl acetate extract of the plant was found to have high cytotoxic activity.