Preparation of a Poly(ethylene glycol)-Based Cross-Linked Network from a Click Reaction for Enzyme Immobilization


Oktay B. , Demir S. , Kayaman Apohan N.

CHEMISTRYSELECT, vol.4, no.20, pp.6055-6059, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 20
  • Publication Date: 2019
  • Doi Number: 10.1002/slct.201900296
  • Title of Journal : CHEMISTRYSELECT
  • Page Numbers: pp.6055-6059
  • Keywords: amylase, click chemistry, enzyme immobilization, thiol-ene, ALPHA-AMYLASE, SUPPORTS, GLYCOL, PEG, FUNCTIONALIZATION, OPTIMIZATION, CONJUGATION, MALEIMIDES, STABILITY, KINETICS

Abstract

In this study, a polyethylene glycol (PEG)-maleimide film was prepared by a thiol-ene click reaction. The covalent immobilization of amylase was carried out onto the PEG-maleimide based photo-curable network via epoxy and C=O groups without any linker. The epoxy and carbonyl groups on the PEG backbone improved the affinity of the enzyme. The immobilization yield of amylase onto the film was 72.46%. The immobilization capacity was also determined as 313 mg/g. The thermal stability of amylase improved via covalent attachment. Immobilized amylase showed maximum activity at 40 degrees C and pH 6.5. The immobilized amylase continued to retain 66% of its initial activity after 30 days.