A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds


Ulag S., ŞAHİN A. , Guncu M. M. , AKSU M. B. , EKREN N. , ŞENGÖR M. , ...More

Bioprinting, vol.24, 2021 (Refereed Journals of Other Institutions) identifier

  • Publication Type: Article / Article
  • Volume: 24
  • Publication Date: 2021
  • Doi Number: 10.1016/j.bprint.2021.e00173
  • Title of Journal : Bioprinting
  • Keywords: 3D printing, Corneal dystrophy, Honeycomb, Polymethylmethacrylate, Thiel-behnke, Vancomycin

Abstract

© 2021 Elsevier B.V.Thiel-Behnke corneal dystrophy, or honeycomb corneal dystrophy, is an autosomal dominant corneal disorder. Tissue engineering can be a novel approach to regenerate this dystrophy. In this study, the honeycomb geometry of the dystrophy mimicked with a 3D printing technology, and 40% PMMA, 40% PMMA/(0.1, 0.5, 2, and 10)% VAN scaffolds were fabricated with honeycomb geometry. As a result of the biocompatibility test with mesenchymal stem cells (MSCs), it can be said that cells on the scaffolds showed high viability and proliferation for all incubation periods. According to the antibacterial activity results, the 40% PMMA/10% VAN showed antibacterial activity against S. aureous. Mechanical results reported that with the addition of VAN into the 40% PMMA, the tensile strength value increased up to 2% VAN amount. The swelling behaviours of the scaffolds were examined in vitro, and found that the swelling rate increased with a high VAN amount. The release of VAN from the scaffolds showed sustained release behaviour, and it took 13 days to be released entirely from the scaffolds.