B cells produce less IL-10, IL-6 and TNF-alpha in myasthenia gravis

Yilmaz V., Oflazer P., Aysal F., Parman Y. G., Direskeneli H., Deymeer F., ...Daha Fazla

AUTOIMMUNITY, cilt.48, sa.4, ss.201-207, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3109/08916934.2014.992517
  • Dergi Adı: AUTOIMMUNITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.201-207
  • Anahtar Kelimeler: Autoantibody, B cell, cytokine, myasthenia gravis, BLOOD MONONUCLEAR-CELLS, MESSENGER-RNA EXPRESSION, REACTIVE T-CELLS, MULTIPLE-SCLEROSIS, RECEPTOR, CYTOKINES, SUBPOPULATIONS, AUTOIMMUNITY, ACTIVATION, ANTIBODIES
  • Marmara Üniversitesi Adresli: Evet

Özet

B cells from myasthenia gravis (MG) patients with autoantibodies (Aab) against acetylcholine receptor (AChR), muscle-specific kinase (MuSK) or with no detectable Aab were investigated as cytokine producing cells in this study. B cells were evaluated for memory phenotypes and expressions of IL-10, IL-6 and IL-12A. Induced productions of IL-10, IL-6, IL-12p40, TNF-alpha and LT from isolated B cells in vitro were measured by immunoassays. MG patients receiving immunosuppressive treatment had higher proportions of memory B cells compared with healthy controls and untreated patients. With CD40 stimulation MG patients produced significantly lower levels of IL-10, IL-6. With CD40 and B cell receptor stimulation of B cells, TNF-alpha production also decreased in addition to these cytokines. The lower levels of these cytokine productions were not related to treatment. Our results confirm a disturbance of B cell subpopulations in MG subgroups on immunosuppressive treatment. B cell derived IL-10, IL-6 and TNF-alpha are down-regulated in MG, irrespective of different antibody productions. Ineffective cytokine production by B cells may be a susceptibility factor in dysregulation of autoimmune Aab production.