Process modelling and simulation of a transketolase mediated reaction: Analysis of alternative modes of operation

Sayar N. A., Chen B. H., Lye G. J., Woodley J. M.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.47, ss.10-18, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.bej.2009.02.014
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.10-18
  • Anahtar Kelimeler: Biocatalysis, Feeding strategy, Mathematical modelling, Simulation, Transketolase, Process analysis, ESCHERICHIA-COLI TRANSKETOLASE, DEPENDENT ENZYMES, PROCESS DESIGN, BIOCATALYSIS, KINETICS, BIOCONVERSION, SELECTION, MYTHS
  • Marmara Üniversitesi Adresli: Hayır

Özet

A previously proposed model of a transketolase catalysed carbon-carbon bond formation reaction condensing beta-hydroxypyruvate and glycolaldehyde to synthesise L-erythrulose has been extended to describe various modes of operation as an alternative to a batch process. The alternative continuous and fed-batch operations, with each substrate being fed separately and together have been analysed using the extended model. The analysis was carried out simulating the product concentration after a given time under defined process conditions. Comparison of product concentration and yield on catalyst as two process metrics were used to identify promising cases for further process development. (C) 2009 Elsevier B.V. All rights reserved.