Determination of catechol O-methyltransferase polymorphisms on patients with essential hypertension


Albar G., Orun O.

International Molecular Medicine Symposium by the Bosphorus, İstanbul, Türkiye, 16 - 18 Mayıs 2019, ss.79

  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.79

Özet

Objective: Catechol O-methyltransferase (COMT) is a ubiquitous enzyme that catalyses the transfer

of a methyl group to catechol-containing neurotransmitters such as dopamine, epinephrine and

norepinephrine. The association of genetic variants of COMT with essential hypertension (EHT) has

been previously shown in some populations. Despite the positive results, the number of studies in

this area is extremely limited. Therefore, in our study, we aimed to determine distribution of a

promoter gene variant in Turkish population and to reveal its correlation with EHT.

Materials-Methods: DNA was isolated from peripheral blood samples (5 ml) of 221 patients

diagnosed with essential hypertension (EHT) and 170 healthy persons without EHT, referred to the

Department of Internal Medicine, Pendik Education and Research Hospital of Marmara University

Medical Faculty. Real-time PCR analyzes were performed using LightCycler 96 (Roche) for

detection of COMT G- 1947A (rs4680) genotypes. Distribution of genotypes and their relation with

EHT was evaluated statistically using GraphPad Prism software (v.7.0).

Results: The genotype distribution for COMT G-1947A (rs4680) variance were found as 88

(39.8%) AA, 76 (34.3%) GA, and 57 (25.9%) GG in 221 EHT patients. Corresponding values for

healthy individuals were 53 (31.1%) AA, 46 (27.1%) GA and 71 (41.8%) GG.

Conclusion: According to our results, G-1947A variance have a strong positive association with

hypertension. Therefore G-1947A polymorphism could be an important risk marker for Turkish

population.