International Molecular Medicine Symposium by the Bosphorus, İstanbul, Türkiye, 16 - 18 Mayıs 2019, ss.79
Objective: Catechol O-methyltransferase (COMT) is a ubiquitous enzyme that catalyses the transfer
of a methyl group to catechol-containing neurotransmitters such as dopamine, epinephrine and
norepinephrine. The association of genetic variants of COMT with essential hypertension (EHT) has
been previously shown in some populations. Despite the positive results, the number of studies in
this area is extremely limited. Therefore, in our study, we aimed to determine distribution of a
promoter gene variant in Turkish population and to reveal its correlation with EHT.
Materials-Methods: DNA was isolated from peripheral blood samples (5 ml) of 221 patients
diagnosed with essential hypertension (EHT) and 170 healthy persons without EHT, referred to the
Department of Internal Medicine, Pendik Education and Research Hospital of Marmara University
Medical Faculty. Real-time PCR analyzes were performed using LightCycler 96 (Roche) for
detection of COMT G- 1947A (rs4680) genotypes. Distribution of genotypes and their relation with
EHT was evaluated statistically using GraphPad Prism software (v.7.0).
Results: The genotype distribution for COMT G-1947A (rs4680) variance were found as 88
(39.8%) AA, 76 (34.3%) GA, and 57 (25.9%) GG in 221 EHT patients. Corresponding values for
healthy individuals were 53 (31.1%) AA, 46 (27.1%) GA and 71 (41.8%) GG.
Conclusion: According to our results, G-1947A variance have a strong positive association with
hypertension. Therefore G-1947A polymorphism could be an important risk marker for Turkish