Genetic diversity and phylogenetic analyses of Turkish sweet corn (Zea mays var. saccharata) varieties using ISSR markers and chloroplast trnL-F IGS region

Filiz E., Uras M. E., Öztürk N., Güngör H., Özyiğit İ. İ.

NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, cilt.52, sa.2, ss.1-14, 2024 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 2
  • Basım Tarihi: 2024
  • Doi Numarası: 10.15835/nbha52213551
  • Dergi Adı: NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aqualine, CAB Abstracts, Pollution Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.1-14
  • Marmara Üniversitesi Adresli: Evet

Özet

 

Genetic diversity levels are critical for characterizing and utilizing germplasm collections and for making improvements  related  to  elite  germplasms.  The  current  study  investigated  the  genetic  diversity  level  and phylogenetic relationships in ten Turkish sweet corn varieties (Zea mays var. saccharata) using 15 ISSR markers and trnL-F intergenic spacer regions, respectively. A total of 75 loci were identified, of which 57 (76%) were polymorphic. The highest polymorphism ratio (100%) was found using UBC811, UBC817, and UBC823 ISSR markers, while the lowest ratio (45.4%) was identified using UBC829. According to trnL-F intergenic spacer  region  analyses,  nucleotide  diversity  was  found  as  π:  0.030  for  Nei  and  θ:  0.036  for  Watterson,respectively. In trnL-F intergenic spacer regions, several polymorphic (variable) sites were identified 28 of which 57% (16/28) were parsimony informative sites and 399 sites were invariable (monomorphic). The phylogenetic analysis revealed that two major groups were observed named groups A and B and ten sweet corn genotypes  clustered  along  with  known  maize  genotypes  in  subgroup  B2  with  98%  bootstrap  value. Consequently,  the  ISSR  data  obtained  in  this  study revealed  that  Turkish  sweet  corn  genotypes  exhibit extensive genetic diversity, and the trnL-F intergenic spacer region was successfully utilized to differentiate between maize genotypes from various origins and whole plant taxa.d utilizing germplasm collections and for making improvements  related  to  elite  germplasms.  The  current  study  investigated  the  genetic  diversity  level  and phylogenetic relationships in ten Turkish sweet corn varieties (Zea mays var. saccharata) using 15 ISSR markers and trnL-F intergenic spacer regions, respectively. A total of 75 loci were identified, of which 57 (76%) were polymorphic. The highest polymorphism ratio (100%) was found using UBC811, UBC817, and UBC823 ISSR markers, while the lowest ratio (45.4%) was identified using UBC829. According to trnL-F intergenic spacer  region  analyses,  nucleotide  diversity  was  found  as  π:  0.030  for  Nei  and  θ:  0.036  for  Watterson,respectively. In trnL-F intergenic spacer regions, several polymorphic (variable) sites were identified 28 of which 57% (16/28) were parsimony informative sites and 399 sites were invariable (monomorphic). The phylogenetic analysis revealed that two major groups were observed named groups A and B and ten sweet corn genotypes  clustered  along  with  known  maize  genotypes  in  subgroup  B2  with  98%  bootstrap  value. Consequently,  the  ISSR  data  obtained  in  this  study revealed  that  Turkish  sweet  corn  genotypes  exhibit extensive genetic diversity, and the trnL-F intergenic spacer region was successfully utilized to differentiate between maize genotypes from various origins and whole plant taxa.izing and utilizing germplasm collections and for making improvements  related  to  elite  germplasms.  The  current  study  investigated  the  genetic  diversity  level  and phylogenetic relationships in ten Turkish sweet corn varieties (Zea mays var. saccharata) using 15 ISSR markers and trnL-F intergenic spacer regions, respectively. A total of 75 loci were identified, of which 57 (76%) were polymorphic. The highest polymorphism ratio (100%) was found using UBC811, UBC817, and UBC823 ISSR markers, while the lowest ratio (45.4%) was identified using UBC829. According to trnL-F intergenic spacer  region  analyses,  nucleotide  diversity  was  found  as  π:  0.030  for  Nei  and  θ:  0.036  for  Watterson,respectively. In trnL-F intergenic spacer regions, several polymorphic (variable) sites were identified 28 of which 57% (16/28) were parsimony informative sites and 399 sites were invariable (monomorphic). The phylogenetic analysis revealed that two major groups were observed named groups A and B and ten sweet corn genotypes  clustered  along  with  known  maize  genotypes  in  subgroup  B2  with  98%  bootstrap  value. Consequently,  the  ISSR  data  obtained  in  this  study revealed  that  Turkish  sweet  corn  genotypes  exhibit extensive genetic diversity, and the trnL-F intergenic spacer region was successfully utilized to differentiate between maize genotypes from various origins and whole plant taxa.