Aim: Methacrylate (MA), is widely used as a monomer in dentistry as well as medicine. MA derivatives have been used for a long time in biomedical devices, in restorative dental composites as well as contact lens materials also in bone cement. Zebrafish has become a popular used model organism in toxicity potential testing. Our aim in this study was to evaluate the effects of a MA derivative, polyethylmethacrylate (PEMA) exposure focusing on Nitric oxide (NO) and development of zebrafish embryo.
Materials and Method: Adult AB strain zebrafish were used in this study that were housed in aquarium rack system (Zebtec, Tecniplast, Italy) at 28 ± 1 °C maintained under a light/dark cycle between 14/10 h. Spherical embryos that were dividing normally were chosed and used for the analysis. Range-finding was applied and after that the zebrafish embryos were exposed to MA in well plates containing 20 embryos, having four replicates. Developmental effects and mortality rate were evaluated for 120h. NO levels of the embryos were evaluated using Griess Method.
Results: In present study no significant difference was observed in the NO levels of the embryos in the control group and in the MA exposed group. However, some developmental defects were observed in some of the MA exposed embryos. Lack of pigmentation was evident in one Dimethyl sulfoxide (DMSO) exposed embryo and pericardial edema was observed in some of the MA exposed embryos.
Conclusions: Our findings suggest that zebrafish embryos are useful models for the assessment of toxicity of dental MA and more research is necessary to determine the potential effects of MA exposure in embryos.