Micro-milling is a micro-mechanical cutting method used to obtain complex and three-dimensional micro geometries. Micro-cutting tools are used in the manufacturing of micro-components and the type of workpiece is also important for good surface quality and minimum burr. In this study, micro machinability of Ti6Al4V alloy which is used most frequently in micro-component production is compared with Ti5553 alloy. Micro-milling of Ti5553 alloy and comparison of the minimum chip thickness with Ti6Al4V were performed for the first time in this study. Using different cutting parameters, the variation of surface roughness, burr width, and cutting forces were investigated. The cutting tests were carried out on a specially designed and high-precision micro-milling test system using a TiCN-coated two-flute end mill of 0.6 mm diameter. According to the results, minimum chip thickness is approximately 0.3 times the edge radius of the cutting tool and does not vary with the alloy type. At feed rates smaller than the minimum chip thickness, both the cutting forces increase and the surface quality decreases. For both alloys, reduced feed rate and increased depth of cut lead to increased burr width. The burr widths in Ti6Al4V alloy are higher. At the end of the study, the limits of the cutting parameters where plowing occurred for the both alloys are clearly determined. In addition, the limits of the cutting parameter causing plowing have been confirmed by cutting forces, surface roughness, and burr formation.