The impact of in vivo Calmette-Guerin Bacillus administration on in vitro IgE secretion in atopic children


Barlan I., Tukenmez F., Bahceciler N., Basaran M.

JOURNAL OF ASTHMA, cilt.39, sa.3, ss.239-246, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 39 Konu: 3
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1081/jas-120002473
  • Dergi Adı: JOURNAL OF ASTHMA
  • Sayfa Sayıları: ss.239-246

Özet

To investigate whether a preexisting T helper (T-H) 2 type immune response could be suppressed by Calmette-Guerin Bacillus (BCG) immunization in atopic children with asthma, we determined interferon (IFN)-gamma interleukin (IL)-2, IL-4, and IL-5 and total IgE level in the supernatant of peripheral blood mononuclear cells (PBMC) of six atopic and five nonatopic children in response to phytohemagglutinin A (PHA), purified protein derivate (PPD), and Dermatophagoides pteronyssinus II allergen (Der p 11) both before and after BCG vaccination. IL-5 level in response to Der p II was significantly higher ill the atopic group than in the nonatopic group both before and after BCG vaccination (p = 0.004, p = 0.009, respectively). In the atopic group, IgE levels determined in PPD and Der p II stimulated and unstimulated culture supernatants decreased significantly after BCG vaccination (p=0.028, p=0.026, p=0.046, respectively), whereas in the "nonatopic group (p = 0.041) BCG vaccination resulted in a significant decrease in IgE level only in response to Der p 11 stimulation. We concluded that in vivo BCG administration can downregulate both spontaneous and stimulated in vitro IgE secretion from PBMC of atopic children.