Mesoscopic tomography imaging of reporter genes in thick printed tissue constructs

ÖZTÜRK M. S., Lee V. K., Zhao L., Dai G., Intes X.

Conference on Diffuse Optical Imaging IV, Munich, Almanya, 12 - 14 Mayıs 2013, cilt.8799 identifier identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 8799
  • Doi Numarası: 10.1117/12.2032539
  • Basıldığı Şehir: Munich
  • Basıldığı Ülke: Almanya
  • Anahtar Kelimeler: Fluorescence tomography, tissue engineering, gene reporter, 3D reconstruction, inverse problem, FLUORESCENCE MOLECULAR TOMOGRAPHY, MICROSCOPY
  • Marmara Üniversitesi Adresli: Hayır

Özet

We report an application of Mesoscopic Fluorescence Molecular Tomography to 3D tissue engineering construct. Engineered thick tissue was hosting two 3D printed vasculatures. The channels were formed by live cells, expressing GFP and mCherry reporter genes, embedded in 3mm turbid media. Tissue and cells kept in a 3mm thick perfusion chamber during the entire imaging process which took less than 5 minutes.