Genome-wide identification and expression profiling of EIL gene family in woody plant representative poplar (Populus trichocarpa)

FİLİZ E., Vatansever R., ÖZYİĞİT İ. İ., Uras M. E., Sen U., Anjum N. A., ...Daha Fazla

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, cilt.627, ss.30-45, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 627
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.abb.2017.06.012
  • Dergi Adı: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.30-45
  • Anahtar Kelimeler: Ethylene, Sulfur, Phytohormone, Poplar, SLIM1, Transcription factor, TRANSCRIPTION FACTORS, MOLECULAR CHARACTERIZATION, ETHYLENE RESPONSES, ARABIDOPSIS SLIM1, EIN3, SULFUR, CLONING, GROWTH, ETHYLENE-INSENSITIVE3, NITROGEN
  • Marmara Üniversitesi Adresli: Evet

Özet

This study aimed to improve current understanding on ethylene-insensitive 3-like (EIL) members, least explored in woody plants such as poplar (Populus trichocarpa Torr. & Grey). Herein, seven putative EIL members were identified in P. trichocarpa genome and were roughly annotated either as EIN3-like sequence associated with ethylene pathway or EIL3-like sequences related with sulfur (S)-pathway. Motif-distribution pattern of proteins also corroborated this annotation. They were distributed on six chromosomes (chrl, 3, 4 and 8-10), and were revealed to encode a protein of 509-662 residues with nuclear localization. The presence of ethylene insensitive 3 (EIN3; PF04873) domain (covering first 80 280 residues from N-terminus) was confirmed by Hidden Markov Model-based search. The first half of EIL proteins (similar to 80-280 residues including EIN3 domain) was substantially conserved. The second half (-300-600 residues) was considerably diverged. Additionally, first half of proteins harbored acidic, praline-rich and glutamine-rich sites, and supported the essentiality of these regions in the transcriptional-activation and protein-function. Moreover, identified six segmental and one-tandem duplications demonstrated the negative or purifying selective nature of mutations. Furthermore, expression profile analysis indicated the possibility of a crosstalk between EIN3-and EIL3-like genes, and co-expression networks implicated their interactions with very diverse panels of biological molecules. (C) 2017 Elsevier Inc. All rights reserved.