Genome-wide identification and expression profiling of EIL gene family in woody plant representative poplar (Populus trichocarpa)

FİLİZ E., Vatansever R., ÖZYİĞİT İ. İ. , Uras M. E. , Sen U., Anjum N. A. , et al.

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, cilt.627, ss.30-45, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 627
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/
  • Sayfa Sayısı: ss.30-45


This study aimed to improve current understanding on ethylene-insensitive 3-like (EIL) members, least explored in woody plants such as poplar (Populus trichocarpa Torr. & Grey). Herein, seven putative EIL members were identified in P. trichocarpa genome and were roughly annotated either as EIN3-like sequence associated with ethylene pathway or EIL3-like sequences related with sulfur (S)-pathway. Motif-distribution pattern of proteins also corroborated this annotation. They were distributed on six chromosomes (chrl, 3, 4 and 8-10), and were revealed to encode a protein of 509-662 residues with nuclear localization. The presence of ethylene insensitive 3 (EIN3; PF04873) domain (covering first 80 280 residues from N-terminus) was confirmed by Hidden Markov Model-based search. The first half of EIL proteins (similar to 80-280 residues including EIN3 domain) was substantially conserved. The second half (-300-600 residues) was considerably diverged. Additionally, first half of proteins harbored acidic, praline-rich and glutamine-rich sites, and supported the essentiality of these regions in the transcriptional-activation and protein-function. Moreover, identified six segmental and one-tandem duplications demonstrated the negative or purifying selective nature of mutations. Furthermore, expression profile analysis indicated the possibility of a crosstalk between EIN3-and EIL3-like genes, and co-expression networks implicated their interactions with very diverse panels of biological molecules. (C) 2017 Elsevier Inc. All rights reserved.