Dietary cholesterol-induced changes of protein kinase C and the effect of vitamin E in rabbit aortic smooth muscle cells

Sirikci Ö., Ozer N., Azzi A.

ATHEROSCLEROSIS, vol.126, no.2, pp.253-263, 1996 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 126 Issue: 2
  • Publication Date: 1996
  • Doi Number: 10.1016/0021-9150(96)05909-6
  • Journal Name: ATHEROSCLEROSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.253-263
  • Keywords: atherosclerosis, vitamin E, protein kinase C, smooth muscle cells, LOW-DENSITY-LIPOPROTEIN, D-ALPHA-TOCOPHEROL, CARDIOVASCULAR-DISEASE, E CONSUMPTION, ATHEROSCLEROSIS, PROLIFERATION, INHIBITION, ARTERIOSCLEROSIS, PATHOGENESIS, LESIONS
  • Marmara University Affiliated: Yes

Abstract

The changes occurring in smooth muscle cells during the development of atherosclerosis in rabbits fed 2% cholesterol and the effect of vitamin E treatment were investigated. Ex-vivo smooth muscle cells obtained from the aorta of cholesterol-fed rabbits exhibited a 2-fold increase of protein kinase C expression and activity. The cholesterol induced changes in protein kinase C were equally present in the membrane bound and cytosolic fraction of the enzyme. The amount of a control protein cc-actin was not affected in smooth muscle cell by the high cholesterol diet treatment, indicating that protein kinase C increase was specific. The increase of protein kinase C expression and activity was not significantly affected by vitamin E treatment although a constant trend was noted. The data are discussed in the light of previous smooth muscle cell in vitro experiments.