Heterologous expression and characterization of a high redox potential laccase from Coriolopsis polyzona MUCL 38443


Pinar O., Tamerler C., Yazgan Karatas A.

TURKISH JOURNAL OF BIOLOGY, cilt.41, sa.2, ss.278-295, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.3906/biy-1605-51
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.278-295
  • Anahtar Kelimeler: Coriolopsis polyzona, enzyme activity, heterologous expression, laccase, Pichia pastoris, purification, TRAMETES-VERSICOLOR LACCASE, LIGNIN-MODIFYING ENZYMES, MOLECULAR-CLONING, GANODERMA-LUCIDUM, FUNGAL LACCASE, GENE, MELANOCARPUS, OPTIMIZATION, PURIFICATION, COPPER
  • Marmara Üniversitesi Adresli: Evet

Özet

In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 mM CuSO4, and 0.6% L-alanine supplementation. The recombinant laccase was partially purified and the molecular weight was found as approximately 54 kDa. The maximum oxidation activity was observed for 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at pH 3.0. The optimal temperature was found as 70 degrees C. On the other hand, at 30 degrees C, the enzyme was stable for more than a week and its half-life was longer than 8 h. The K-m, V-max, k(cat), and k(cat) K-m(-1) values of the recombinant laccase were identified as 0.137 mM, 288.6 mu mol min(-1) L-1, 5.73 x 10(5) min(-1), and 4.18 x 10(6) min(-1) mM(-1), respectively. Sodium azide, L-cysteine, and SDS were found as usual inhibitors.