Particle bombardment technology and its applications in plants

ÖZYİĞİT İ. İ., Yucebilgili Kurtoglu K.

MOLECULAR BIOLOGY REPORTS, cilt.47, sa.12, ss.9831-9847, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 47 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s11033-020-06001-5
  • Dergi Adı: MOLECULAR BIOLOGY REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.9831-9847
  • Anahtar Kelimeler: Biolistic, Gene gun, Direct gene transfer, Transgenic plants, Gene delivery, Genetic transformation, TRANSIENT GENE-EXPRESSION, SOYBEAN GLYCINE-MAX, STABLE TRANSFORMATION, TRANSGENIC PLANTS, BIOLISTIC TRANSFORMATION, MEDIATED TRANSFORMATION, ABSCISIC-ACID, FOREIGN GENES, DNA DELIVERY, RICE
  • Marmara Üniversitesi Adresli: Hayır

Özet

Particle bombardment, or biolistics, has emerged as an excellent alternative approach for plant genetic transformation which circumvents the limitations of Agrobacterium-mediated genetic transformation. The method has no biological constraints and can transform a wide range of plant species. Besides, it has been the most efficient way to achieve organelle transformation (for both chloroplasts and mitochondria) so far. Along with the recent advances in genome editing technologies, conventional gene delivery tools are now being repurposed to deliver targeted gene editing reagents into the plants. One of the key advantages is that the particle bombardment allows DNA-free gene editing of the genome. It enables the direct delivery of proteins, RNAs, and RNPs into plants. Owing to the versatility and wide-range applicability of the particle bombardment, it will likely remain one of the major genetic transformation methods in the future. This article provides an overview of the current status of particle bombardment technology and its applications in the field of plant research and biotechnology.