Determination of Natamycin in Turkish Yoghurt


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Bilğiç Alkaya D. , Karalomlu O.

INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY, 2016 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2016
  • Doi Number: 10.1155/2016/8480963
  • Title of Journal : INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY

Abstract

This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150mm x 4.6mm x 5 mu m) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented.