Akademik Veri Yönetim Sistemi
The 13th International Symposium on Pharmaceutical Sciences (ISOPS). , Ankara, Türkiye, 22 - 25 Temmuz 2021, sa.129, ss.83-84
EVALUATION OF ANTIOXIDANT,
ANALGESIC AND ANTIINFLAMMAUTORY ACTIVITY AND GENOTOXIC EFFECT OF MICROMERIA
FRUTICOSA SUBSP BRACHYCALYX IN VITRO AND IN VIVO
1Celikkol
I., 2Beceren A., 3Kabasakal
L., 4Taskin T., 5Aydemir S.
1Marmara University, Institute of Health
Sciences, Department of Pharmaceutical Toxicology Istanbul, Turkey, celikkolisik@gmail.com
2Marmara University, Department of
Pharmaceutical Toxicology, Istanbul, Turkey, ayfertozan@hotmail.com
3Marmara University, Department of
Pharmacology, Istanbul, Turkey, lkabasakal@gmail.com
4Marmara University, Department of
Pharmacognosy, Istanbul, Turkey, turguttaskin@marmara.edu.tr
5Marmara University, Vocational School of
Health Services, Department of Medical Services and Techniques, Istanbul,
Turkey, szgnaydemir@gmail.com
Introduction: Today, antioxidants have been
using diversely in various medical conditions. Therefore, there are growing
interest for discovering and developing more effective and safer antioxidants
derived from natural sources. Certain Micromeria species were identified as a
rich source of antioxidant agents (1). This study aimed to investigate possible
antioxidant activity and genotoxicity of Micromeria fruticosa subsp brachycalyx
in methanol extract both in vitro and in vivo.
Materials
and Methods: In
vitro antioxidant activity of Micromeria fruticosa subsp brachycalyx in
methanol extract was evaluated by DPPH, ABTS, FRAP and CUPRAC assays. In
vivo analyses performed on Balb/c mice that divided to three groups (n=6
for each group) as control group (%0,04 Carboxymethyl cellulose solution (CMC),
0,1 mL/10 g, p.o.), positive control group (Indomethacin in %0,04 CMC, 10
mg/kg, p.o.) and treatment group (Micromeria extract in %0,04 CMC, 200
mg/kg, p.o.) and each regimen was applied for 10 days. Myeloperoxidase levels
were analyzed in mice liver and kidney tissues. Genotoxic effect was determined
in mice blood with Comet Technique. The 8-hydroxy-2’-deoxyguanosine was
analyzed in mice liver and kidney tissues with commercial ELISA kit.
Results: The results showed that plant
extract showed stronger DPPH radical scavenging activity, however, lower ABTS, CUPRAC
and FRAP values versus to standards. According to the graph, which shows the
elapsed time (second) in pain behavior versus time (minute) for each group,
treatment group exhibited its efficacy in late phase (20-30 min) similar with
indomethacin group. Myeloperoxidase levels in liver tissues of the extract
group were significantly lower compared to the control group (p<0.05).
Myeloperoxidase levels in kidney tissues of the extract group were not
significantly different compared to both control and indomethacin group. Micromeria
fruticosa subsp brachycalyx extract exerted no genotoxic effect on DNA
similarly both control and indomethacin group.
Conclusions: Our study showed that Micromeria
fruticosa subsp brachycalyx has a potential antioxidant activity. It has
also been contributed to raise awareness to this species in terms of its safety
with the obtained results. Therefore, the usage of this species as natural
antioxidant source is promising for new drug candidates.
Acknowledgements:
Not
available.
References:
1. Salameh N et al., (2020) Screening of
Antioxidant and Antimicrobial Activity of Micromeria fruticosa serpyllifolia
Volatile Oils: A Comparative Study of Plants Collected from Different Regions
of West. BioMed Research International, 1-7.