Sequential Triplex Real-Time PCR Assay for Detecting 21 Pneumococcal Capsular Serotypes That Account for a High Global Disease Burden


Pimenta F. C. , Roundtree A., Soysal A., Bakir M., du Plessis M., Wolter N., ...Daha Fazla

JOURNAL OF CLINICAL MICROBIOLOGY, cilt.51, sa.2, ss.647-652, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 51 Konu: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1128/jcm.02927-12
  • Dergi Adı: JOURNAL OF CLINICAL MICROBIOLOGY
  • Sayfa Sayıları: ss.647-652

Özet

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.