Sequential Triplex Real-Time PCR Assay for Detecting 21 Pneumococcal Capsular Serotypes That Account for a High Global Disease Burden


Pimenta F. C. , Roundtree A., Soysal A., Bakir M., du Plessis M., Wolter N., ...More

JOURNAL OF CLINICAL MICROBIOLOGY, vol.51, no.2, pp.647-652, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 2
  • Publication Date: 2013
  • Doi Number: 10.1128/jcm.02927-12
  • Title of Journal : JOURNAL OF CLINICAL MICROBIOLOGY
  • Page Numbers: pp.647-652

Abstract

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.