Identification of Molecular Network of Gut-Brain Axis Associated with Neuroprotective Effects of PPARδ-Ligand Erucic Acid in Rotenone-Induced Parkinson's Disease Model in Zebrafish.


Ünal İ., Cansız D., Sürmen M. G., Sürmen S., Sezer Z., Beler M., ...Daha Fazla

The European journal of neuroscience, cilt.57, ss.585-606, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1111/ejn.15904
  • Dergi Adı: The European journal of neuroscience
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Psycinfo, Veterinary Science Database
  • Sayfa Sayıları: ss.585-606
  • Anahtar Kelimeler: gut-brain axis, LC-MS/MS analyses, Parkinson's disease, rotenone, zebrafish, omega 9-fatty acids, PROLIFERATOR-ACTIVATED-RECEPTOR, AGONIST PROVIDES NEUROPROTECTION, OXIDATIVE STRESS, SUBSTANTIA-NIGRA, PINK1, COMPLEX, DJ-1, MITOCHONDRIA, CALRETININ, PARAMETERS
  • Marmara Üniversitesi Adresli: Evet

Özet

Disruption of the gut-brain axis in Parkinson's disease (PD) may lead to motor symptoms and PD pathogenesis. Recently, the neuroprotective potential of different PPAR delta-agonists has been shown. We aimed to reveal the effects of erucic acid, peroxisome proliferator-activated receptors (PPARs)-ligand in rotenone-induced PD model in zebrafish, focusing on the gut-brain axis. Adult zebrafish were exposed to rotenone and erucic acid for 30 days. Liquid chromatography-mass spectrometry and tandem mass spectrometry (LC-MS/MS) analysis was performed. Raw files were analysed by Proteome Discoverer 2.4 software; peptide lists were searched against Danio rerio proteins. STRING database was used for protein annotations or interactions. Lipid peroxidation (LPO), nitric oxide (No), alkaline phosphatase, superoxide dismutase, glutathione S-transferase (GST), acetylcholinesterase and the expressions of PD-related genes were determined. Immunohistochemical tyrosine hydroxylase (TH) staining was performed. LC-MS/MS analyses allowed identification of over 2000 proteins in each sample. The 2502 and 2707 proteins overlapped for intestine and brain. The 196 and 243 significantly dysregulated proteins in the brain and intestines were found in rotenone groups. Erucic acid treatment corrected the changes in the expression of proteins associated with cytoskeletal organisation, transport and localisation and improved locomotor activity, expressions of TH, PD-related genes (lrrk2, park2, park7, pink1) and oxidant-damage in brain and intestines in the rotenone group as evidenced by decreased LPO, No and increased GST. Our results showed beneficial effects of erucic acid as a PPAR delta-ligand in neurotoxin-induced PD model in zebrafish. We believe that our study will shed light on the mechanism of the effects of PPAR delta agonists and omega 9-fatty acids in the gut-brain axis of PD.