The efficacy of probiotic mediated hydroxyapatite in mammalian cell culture: promotion of MG–63 and L–929, anti–MCF7 breast cancer cells activities, and cytokine production of PBMC

Nouri S., Roghanian R., Emtiazi G., GÜNDÜZ O., Shafiei R.

Emergent Materials, 2024 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s42247-024-00828-5
  • Dergi Adı: Emergent Materials
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Anahtar Kelimeler: Anti-inflammatory, Anticancer, Bone formation, Hydroxyapatite, Probiotics
  • Marmara Üniversitesi Adresli: Evet

Özet

Due to the probiotic characteristics of Bacillus subtilis and Bacillus coagulans, bacterial debris not only strengthens the immune system but also qualifies as a merit for biomedical products like toothpaste and implants for Hydroxyapatite (HA) synthesis. Since synthetic HA has caused some disadvantages, including raising proinflammatory cytokines like interleukin-6, the current study investigates the effects of bio-mediated HA synthesis from Bacillus subtilis and Bacillus coagulans on cell proliferation. As per the flow cytometry and MTT results, the probiotic HA not only exhibits anticancer activity against MCF-7 but also enhances the viability of L-929, PBMC, and MG-63 cells. Additionally, it promotes osteogenic mineralization, a finding supported by alkaline phosphatase activity, alizarin red staining, and the real-time PCR assay. It should be mentioned that this probiotic debris-mediated HA not only elevate the healthy cells’ viability but also regulate the cytokine secretion in order to facilitate immune system functionality and adaptation. The data suggest that this bioproduct is proposed for cancer therapy, bone development, and sanitation. Graphical Abstract: (Figure presented.)