Determination of spatial proximity between the N-terminus and cocaine binding site of the dopamine transporter by FRET

ORUN O., MEGA TİBER P.

MARMARA MEDICAL JOURNAL, cilt.28, sa.3, ss.123-128, 2015 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 3
  • Basım Tarihi: 2015
  • Doi Numarası: 10.5472/mmjoa.2803.01
  • Dergi Adı: MARMARA MEDICAL JOURNAL
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.123-128
  • Marmara Üniversitesi Adresli: Evet

Özet

Objective: The dopamine transporter (DAT) mediates uptake of dopamine from the synaptic cleft and provides rapid termination of neurotransmission. It is the site of action for different drugs of abuse, including cocaine and amphetamine. Serine 7 and 12 at the N-terminus were found to be critical residues in phosphorylation. Here, we addressed spatial proximity site relationship of N-terminal extension with respect to cocaine binding in wild type and Ser7/12Asp and Ser7/12Ala mutants.