Iron, which is an indispensable metal due to its low cost and high durability, is used in many fields from food sector to automotive industry, from home appliances to stationery materials. It is also a biologically essential component of all living organisms. When iron ions are present in cells in excess or in low amounts, many diseases can occur. For these reasons, determination of Fe(III) ions is very important. So far, the methods in used for determination of iron are voltammetry, capillary electrophoresis, inductively coupled plasma mass spectrometry (ICP-MS), inductively coupled plasma mass spectrometry (ICP-MS) and flame atomic absorption spectroscopy (FAAS) etc. But almost all of these methods are time taking methods due to complicated and multi stepped sample preparation procedure. In this study, a new method for the determination of Fe(III) ions using a polymeric membrane prepared by photopolymerization technique has been developed. The structural and morphological properties of the membrane were studied and then the parameters such as pH, calibration range, sensitivity, selectivity, response time and reproducibility were systematically investigated. The developed polymeric sensor was successfully applied to the determination of Fe(III) ions in food and water samples with satisfactory results.