Kinetic analysis of the inhibitory effect of trichloroethylene (TCE) on nitrification in cometabolic degradation


KOCAMEMİ B., ÇEÇEN F.

BIODEGRADATION, cilt.18, sa.1, ss.71-81, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/s10532-005-9037-3
  • Dergi Adı: BIODEGRADATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.71-81
  • Marmara Üniversitesi Adresli: Evet

Özet

In this study, the inhibitory effect of TCE on nitrification process was investigated with an enriched nitrifier culture. TCE was found to be a competitive inhibitor of ammonia oxidation and the inhibition constant (K-I) was determined as 666-802 mu g/l. The TCE affinity for the AMO enzyme was significantly higher than ammonium. The effect of TCE on ammonium utilization was evaluated with linearized plots of Monod equation (e.g., Lineweaver-Burk, Hanes-Woolf and Eadie-Hofstee plots) and non-linear least square regression (NLSR). No significant differences were found among these data evaluation methods in terms of kinetic parameters obtained.