Akademik Veri Yönetim Sistemi
17th International Medicine and Heath Sciences Researches Congress UTSAK, Ankara, Türkiye, 19 - 20 Ekim 2024, ss.49-50
Cancer is still a major clinical challenge that lacks a known cure, although research into new targets and therapy possibilities continues unabated. Natural phytochemicals derived from various herbal sources emerge as novel therapeutic agents in oncology due to their enhanced efficacy, 177h UTSAK, 19-20 October 2024, Ankara | 50 selectivity, and reduced toxicity. The Papaver pseudo-orientale (Fedde) medw. plant, which is utilized for a variety of purposes in the public and recent research has generated interest in examining its bioactive components and their impact on human health, was assessed in this study for its cytotoxic and genotoxic properties. Papaver pseudo-orientale (Fedde) medw. was collected from Tunceli in Türkiye. The ethanol extract was derived from the aerial parts of the Papaver pseudo-orientale plant using the maceration technique. The cytotoxic effect of Papaver pseudo-orientale ethanol extract concentrations (10-200 µg/ml) on human prostate cancer cell lines (PC-3) and human breast cancer cell lines (MCF-7) was determined by the MTT assay, and the genotoxic effect was determined by the Comet Assay method. The results indicate that Papaver pseudo-orientale ethanol extract exhibited minimal inhibition over the entire dose range in MCF-7 cells; however, significant inhibition was noted in PC-3 cells at doses of 100 and 200 µg/mL after 24 hours of treatment. At the maximum dose of 200 µg/mL, PC-3 cells exhibited 53.12% inhibition. The MTT findings indicated an IC50 value of 182 µg/mL for PC-3 cells. Following 24 hours of exposure to Papaver pseudo-orientale extract, there was a statistically significant increase in DNA damage in a dose-dependent manner in PC-3 cells. This study provides important information that suggests Papaver pseudo-orientale may play an important role in the cell cycle progression of prostate carcinoma cell lines. We anticipate that this preliminary study will result in the development of a novel medicine for cancer treatment. However, additional research is required to supports these findings.