Akademik Veri Yönetim Sistemi
Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2025 – Winter Edition, Bucuresti, Romanya, 26 - 28 Kasım 2025, ss.150-151, (Özet Bildiri)
In this study, biodegradable methacrylated silk fibroin (SilkMA) scaffolds were developed, fabricated, and comprehensively characterized to evaluate their potential for tissue engineering applications. Silk fibroin was chemically modified with methacrylate groups to obtain a photocurable biomaterial suitable for stereolithographic fabrication. To introduce magnetic responsiveness and enable controlled internal alignment, iron oxide (Fe₃O₄) nanoparticles were homogeneously dispersed within SilkMA hydrogels. This approach aimed to guide polymer chain orientation and create anisotropic architectures that can mimic the hierarchical structure of native tissues. A custom-designed three-axis Helmholtz coil system was engineered and integrated into a 3D stereolithography (SLA) printer, generating a uniform 10 mT magnetic field during the printing process. The synchronized application of light polymerization and magnetic alignment enabled precise control over microstructural organization throughout the scaffold. Comprehensive physicochemical and biological analyses demonstrated that the printed SilkMA scaffolds possessed favorable rheological and electrical conductivity profiles, indicating their suitability for biomedical applications. Fibroblast cells cultured on magnetically oriented scaffolds exhibited more than 90% viability after 1, 3, and 7 days, confirming excellent cytocompatibility and the absence of cytotoxic effects. Scanning electron microscopy (SEM) and DAPI staining revealed enhanced cell adhesion and proliferation on the aligned structures, while atomic force microscopy (AFM) verified the anisotropic microstructural orientation. Mechanical compression, swelling, and degradation tests further showed that the scaffolds displayed high elasticity, stability, and a controlled degradation profile over 14 days. Collectively, these results demonstrate that magnetically assisted 3D printing provides an effective strategy for fabricating anisotropic, biocompatible SilkMA-based scaffolds with tunable mechanical and structural properties.