HPLC analysis of in vivo metabolites of 4-nitrobenzoic acid [(5-nitro-2-thiopheneyl)methylenelhydrazide in rats


Kocyigit-Kaymakcioglu B. , Unsalan S., KÜÇÜKGÜZEL Ş. G. , ŞENER G. , Rollas S.

EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS, cilt.32, sa.4, ss.197-203, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Konu: 4
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/bf03191004
  • Dergi Adı: EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
  • Sayfa Sayıları: ss.197-203

Özet

The in vivo metabolism of 4-nitrobenzoic acid [(5-nitro-2-thiopheneyl)methylenelhydrazide (substrate), a model that represents hydrazide hydrazone compounds, was investigated in the rat. The metabolites were monitored in rat plasma at certain time intervals. The substrate was dissolved in dimethylsulfoxide (DMSO)/water (1:4) and administered intraperitoneally at dose of 100 mg/kg and 500 mg/kg. Blood samples were collected at 30 min, then at 1, 2, 4, 8, 12 and 24 h post-administration. The chromatographic separation of the substrate and its metabolites was performed on a Novapak C 18 (Phenomenex) (150 mm x 4.6 mm i.d., 5-mu m particle size) using a mobile phase consisting of phosphate buffer: acetonitrile (90: 10, v/v) with a linear gradient system. From the biotransformation of this compound, 4-nitrobenzoic acid (M3) was identified together with the substrate, as evidenced by high pressure liquid chromatography (HPLC)-UV/ diode array detection (DAD).