Akademik Veri Yönetim Sistemi
12TH INTERNATIONAL İSTANBUL SCIENTIFIC RESEARCH CONGRESS, İstanbul, Türkiye, 29 - 31 Mayıs 2024, (Yayınlanmadı)
Bacillus cereus, a Gram-positive bacterium, is one of the most common causes of food poisoning outbreaks. It has a pathogenic effect on many foods, especially starchy foods, vegetables, dairy products, bread, and meat products. Its ability to form biofilms causes serious problems in the food industry. Chemical cleaning agents are often used to eliminate biofilms; however, these approaches are not always effective. Since synthetic antimicrobial agents can cause adverse effects, interest in discovering natural resource-based antagonists has increased significantly. Plants, in particular, are known as important natural resources with strong antimicrobial effects. Centaurea species have been known to have antipyretic, antidiarrheal, antimicrobial, antioxidant and anti-inflammatory activities and are a good remedy for stomach problems thanks to their rich terpenes, alkaloids and secondary metabolites. Studies have shown that also Campanula lyrata plant is rich in secondary metabolites. This study is aimed to determine the antimicrobial and antibiofilm activities of Centaurea kilaea and Campanula lyrata subsp. lyrata extracts on B. cereus bacteria. For this reason, methanol extracts of Centaurea kilaea and Campanula lyrata subsp. lyrata leaves were used and antibacterial and antibiofilm activities were determined spectrophotometrically using a serial dilution method in a 96-well microplate. As a results of C. kilaea, 2.4, 1.2 mg/ml and 600 µg/ml concentrations were found to have 90.04 ± 0.02, 92.42 ± 0.01 and 91.95 ± 0.01% bacterial inhibition, respectively. In addition, biofilm inhibition of 75 and 37.5 µg/ml concentrations was found to be 59.96 ± 0.06% and 54.94 ± 0.04%, respectively. Campanula lyrata subsp. lyrata extract did not show antibacterial and antibiofilm activity. According to these results, it is suggested that C. kilaea leaf extracts may be a potent antibacterial agent on B. cereus bacteria, a food pathogen, and a natural method that can inhibit the formation of the biofilm structure by B. cereus bacteria.