Prophylactic Role of Oral L-Arginine on Histological and Contractile Changes in a Rat Chronic Bladder Injury Model

Ozgur A., Onol F. F. , ERCAN F. , TARCAN T.

UROLOGIA INTERNATIONALIS, cilt.81, sa.3, ss.347-352, 2008 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 81 Konu: 3
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1159/000151417
  • Sayfa Sayıları: ss.347-352


Introduction: The effect of L-arginine on bladder tissue structure and function in a rat bladder injury model were investigated. Methods: 24 male Sprague-Dawley rats were used. The control group received a regular diet. The L-arginine group received oral L-arginine (1 g/day). The protamine sulfate (PS) group received intravesical PS every 48 h (0.5 ml, 5 mg/ml). L-Arginine was administered to the PS+L-arginine group in addition to PS. At the end of 1 week, bladder tissues were processed for histological and functional studies. Results: The PS group revealed urothelial damage with glycosaminoglycan layer irregularity and mast cell infiltration which was not evident in the PS+L-arginine group. 120 m M potassium and electrical field stimulation (EFS)-induced contractions in the PS group were significantly lower than in other groups, whereas carbachol-induced contractions were not significantly different. Relaxation responses of precon-tracted strips to EFS and isoproterenol did not reveal a significant decrease in the PS group, whereas L-arginine significantly enhanced these responses in PS-treated animals. Conclusions: Intravesical PS causes urothelial damage and inflammation that is associated with significant changes in rat bladder tissue contractility. Oral L-arginine treatment is found to prevent these histological and contractile alterations. Our findings may indicate the nitric oxide-cGMP pathway as a possible therapeutic target in various bladder diseases associated with urothelial damage. Copyright (c) 2008 S. Karger AG, Basel