In vitro investigation of the effects of colistin alone with and tigecycline, imipenem and rifampicin combinations on polymorphonuclear leukocyte functions, oxidative stress, oxidant and antioxidant enzymes in patients with bronchiectasis


GÜRBÜZ B. , Gurer Ü. , Cevik O., Yalcinkaya I., BEKİROĞLU G. N. , Cevikbas A.

MARMARA PHARMACEUTICAL JOURNAL, cilt.18, sa.1, ss.26-35, 2014 (ESCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Konu: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.12991/201414123
  • Dergi Adı: MARMARA PHARMACEUTICAL JOURNAL
  • Sayfa Sayıları: ss.26-35

Özet

In our study was investigated the in vitro effects of Colistin(4 mu g/ml), alone with the combination of tigecycline(0.87 mu g/ml), imipenem (30 mu g/ml) and rifampicin (7 mu g/ml) at therapeutic concentrations and malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) activity on polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) of patients with bronchiectasis. PMNLs were isolated from venous blood by Ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander's method. MDA levels were determinated by Beuge's method, SOD, GSH-Px and MPO activities were assayed by Sun's, Paglia and Valentine's and Hillegas's, methods respectively. All results are statistically evaluated. Phagocytic (p<0.0001), and intracellular killing activity (p=0.0113, p=0.0008, p=0.0014, p=0.0036) in the PMN of patients with bronchiectasis significantly increased when compared to the control. Enzyme activity (SOD, GSH-Px and MPO) and MDA level significantly decreased (p<0.001) in patients with bronchiectasis when compared to the control.