Microbial sulfate reduction at low (8 °C) temperature using waste sludge as a carbon and seed source


Sahinkaya E.

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, cilt.63, sa.3, ss.245-251, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 3
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.ibiod.2008.09.006
  • Dergi Adı: INTERNATIONAL BIODETERIORATION & BIODEGRADATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.245-251
  • Anahtar Kelimeler: Acid mine drainage, Low temperature, Sulfate reduction, Waste sludge
  • Marmara Üniversitesi Adresli: Hayır

Özet

Biological treatment of sulfate and metal-containing wastewater (such as acid mine drainage) is a viable option due to lower cost and better sludge quality compared to conventional chemical treatment. Although several substrates can be used as carbon source, a low-cost substrate is required for large scale applications. This study was conducted to investigate the suitability of waste sludge as a carbon and seed source for sulfate reduction at 8 °C in batch bioassays. Around 7 mmol of sulfate was reduced when the waste sludge mixture (WS) (6700 mg SS l-1) from primary and secondary settling tank was supplemented as a carbon and seed source. However, only 1.6 mmol of sulfate was reduced with anaerobic digester effluent (ADS) (5300 mg SS l-1). The produced H2S from 1 g VSS l-1 WS and ADS oxidation can theoretically precipitate around 90 and 35 mg Fe2+, respectively. Both WS and ADS oxidized ethanol to acetate at similar rates. It appears that WS is a good candidate for carbon and start-up seed source of sulfate reduction at 8 °C, whereas sulfidogenic acetate oxidation was the limiting step. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that both sludge sources contain Desulfomicrobium apsheronum strain. © 2008 Elsevier Ltd. All rights reserved.