alpha-Amylase immobilization on functionalized nano CaCO3 by covalent attachment

DEMİR S., Gok S. B., KAHRAMAN M. V.

STARCH-STARKE, cilt.64, sa.1, ss.3-9, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/star.201100058
  • Dergi Adı: STARCH-STARKE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3-9
  • Anahtar Kelimeler: a-Amylase, Covalent Immobilization, Enzyme, Glutaraldehyde, Nano CaCO3, ENZYME, PERFORMANCE, STABILITY, CHITOSAN, SURFACE, MATRIX, TOOL
  • Marmara Üniversitesi Adresli: Evet

Özet

In this study, a-amylase was immobilized on glutaraldehyde activated silanized calcium carbonate nanoparticles by a using covalent binding method. The surface modified nano calcium carbonate (CaCO3) were characterized using FTIR and SEM. Immobilization yield was found as 199.43 mg/g of calcium carbonate nanoparticles. The maximum activity was observed at pH 6.5. The immobilized enzyme had a higher activity at elevated temperature (5090 degrees C) than the free one. Reuse studies demonstrated that the immobilized enzyme could reuse 25 times while retaining 18.2% of its activity. Free enzyme lost its activity completely within 15 days. Vmax values for the free and immobilized enzymes were calculated as 10 and 0.35 mg/mL/min, respectively.