alpha-Amylase immobilization on functionalized nano CaCO3 by covalent attachment
STARCH-STARKE, cilt.64, sa.1, ss.3-9, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 64 Sayı: 1
- Basım Tarihi: 2012
- Doi Numarası: 10.1002/star.201100058
- Dergi Adı: STARCH-STARKE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.3-9
- Anahtar Kelimeler: a-Amylase, Covalent Immobilization, Enzyme, Glutaraldehyde, Nano CaCO3, ENZYME, PERFORMANCE, STABILITY, CHITOSAN, SURFACE, MATRIX, TOOL
- Marmara Üniversitesi Adresli: Evet
Özet
In this study, a-amylase was immobilized on glutaraldehyde activated silanized calcium carbonate nanoparticles by a using covalent binding method. The surface modified nano calcium carbonate (CaCO3) were characterized using FTIR and SEM. Immobilization yield was found as 199.43 mg/g of calcium carbonate nanoparticles. The maximum activity was observed at pH 6.5. The immobilized enzyme had a higher activity at elevated temperature (5090 degrees C) than the free one. Reuse studies demonstrated that the immobilized enzyme could reuse 25 times while retaining 18.2% of its activity. Free enzyme lost its activity completely within 15 days. Vmax values for the free and immobilized enzymes were calculated as 10 and 0.35 mg/mL/min, respectively.