Inhibition of autophagy enhances DENSpm-induced apoptosis in human colon cancer cells in a p53 independent manner

Gurkan A., Arisan E. D., Yerlikaya P. O., Ilhan H., Unsal N. P.

CELLULAR ONCOLOGY, cilt.41, sa.3, ss.297-317, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s13402-017-0369-x
  • Dergi Adı: CELLULAR ONCOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.297-317
  • Anahtar Kelimeler: Colon cancer, Apoptosis, Autophagy, Polyamine analogue, DENSpm, HUMAN-MELANOMA CELLS, POLYAMINE ANALOG N-1,N-11-DIETHYLNORSPERMINE, SPERMIDINE/SPERMINE N-1-ACETYLTRANSFERASE, CARCINOMA-CELLS, INDUCTION, RESISTANCE, DEATH, METABOLISM, CATABOLISM, MODULATORS
  • Marmara Üniversitesi Adresli: Hayır

Özet

One of the recently developed polyamine (PA) analogues, N (1) ,N (11)-diethylnorspermine (DENSpm), has been found to act as an apoptotic inducer in melanoma, breast, prostate and colon cancer cells. Also, its potential to induce autophagy has been established. Unfolded protein responses and starvation of amino acids are known to trigger autophagy. As yet, however, the molecular mechanism underlying PA deficiency-induced autophagy is not fully clarified. Here, we aimed to determine the apoptotic effect of DENSpm after autophagy inhibition by 3-methyladenine (3-MA) or siRNA-mediated Beclin-1 silencing in colon cancer cells.