IMMOBILIZATION OF LIPASE ONTO MOF-BASED FILM


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Aslan M. B., Oktay B., Demir S.

IX. INTERNATIONAL HEALTH, ENGINEERING AND SCIENCES CONGRESS, Ankara, Türkiye, 27 - 29 Eylül 2024, ss.245-246

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.245-246
  • Marmara Üniversitesi Adresli: Evet

Özet

Enzymes have an important place in various industrial areas. However, they have some commercial limitations due to various reasons such as the difficulty in reuse of enzymes and the high cost of purification. Enzyme immobilization has been a frequently investigated technique due to its improving effects on various properties of the enzyme, including enzyme reuse and enzyme stability. Lipases (EC 3.1.1.3) are serine hydrolase class enzymes. Lipases have been the subject of many studies due to their usability in different sectors and their ability to recognize various substrates. In this study, the immobilization of lipase enzyme isolated from Candida rugosa onto MOF-based films and its effects on the enzyme were investigated. Therefore, in our study, different formulations of poly(ethylene glycol) dimethacrylate, N-[3(Dimethylamino)propyl] methacrylamide (DMAPMA) and amine modified zeolitic imidazolate framework8 (ZIF8-NH2) were prepared and polymerization was carried out under UV light by adding a photoinitiator. Lipase enzyme was covalently immobilized on MOF-based films and changes in various properties of the enzyme as a result of immobilization were investigated. The results were compared by examining the storage time, kinetic properties, temperature and reusability of free and immobilized enzymes using hydrolysis and synthesis activity methods. The immobilized enzyme showed better results than the free enzyme in terms of hydrolysis and synthesis activity. In addition, the immobilized enzyme showed better reusability and storage stability. This study was supported by Marmara University Scientific Research Project Commission, FYL-2023-10978. Keywords: Lipase, immobilization, ZIF, film, UV light