Cytotoxic, apoptotic and genotoxic effects of thymoquinone‑oxime derivative on gastric cancer cells: An in vitro study

Bozali K., İNce Z., Kiziltoprak N., Gülşen T., Köstek M., Kesgin Y. M., ...Daha Fazla

Oncology Letters, cilt.31, sa.3, 2026 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 3
  • Basım Tarihi: 2026
  • Doi Numarası: 10.3892/ol.2026.15462
  • Dergi Adı: Oncology Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE
  • Anahtar Kelimeler: cytotoxicity, gastric cancer, genotoxicity, oxime, thymoquinone
  • Marmara Üniversitesi Adresli: Evet

Özet

Gastric cancer (GC) remains a notable global health concern, emphasizing the need for novel and effective thera‑ peutic agents. The present study investigated the cytotoxic, genotoxic and apoptotic effects of thymoquinone‑oxime (TQ‑ox) on human gastric adenocarcinoma AGS cells and assessed its potential to induce DNA damage. Cytotoxicity was evaluated in AGS and normal human gastric epithelial cells (HGEpiCs) using a luminometric ATP assay. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were measured fluorometrically, whereas intracellular glutathione (GSH) content was determined via a luminometric GSH assay kit. DNA damage was quantified by a comet assay, and apoptosis was assessed by fluorescence microscopy with acridine orange/ethidium bromide (AO/EB)