Morphological and Biochemical Evaluation of Effects of Myrtus communis L. Extract on Heart and Aorta in High Fat-Diet Induced Obese Rats

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Özyılmaz Yay N., Bülbül Aycı N., Kaya R., Şen A., Şener G., Ercan F.

MARMARA MEDICAL JOURNAL, cilt.36, sa.2, ss.162-170, 2023 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 2
  • Basım Tarihi: 2023
  • Dergi Adı: MARMARA MEDICAL JOURNAL
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.162-170
  • Marmara Üniversitesi Adresli: Evet

Özet

Objectives: The purpose of this study was to examine the protective effects of Myrtus communis L. (MC) extract on high fat-diet (HFD) induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide system (eNOS).

Materials and Methods: Wistar albino male rats were divided into 3 groups (n=7) as control, HFD and HFD+MC. Rats in HFD and HFD+MC groups were HFD fed for 16 weeks and in the last 4 weeks saline or MC (100 mg/kg) was administered orally (5 days/week). Triglyceride, cholesterol and HDL were estimated in blood serum. Tissue oxidative stress and inflammatory parameters were evaluated biochemically. Tissues morphologies, eNOS, inducible NOS (iNOS) and NADPH oxidase-2 (NOX-2)-immunopositive and apoptotic cells were evaluated histologically.

Results: Altered serum lipid profiles, degenerated heart and aorta morphology, increased malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, tumor necrosis factor-alpha, monocyte chemoattractant protein-1 and myeloperoxidase levels and iNOS, NOX-2 immunopositive and apoptotic cells, decreased NO levels, eNOS-immunopositive cells in both tissues were observed in HFD group. All these parameters improved in HFD+MC group.

Conclusion: This study revealed that HFD-induced obesity increased iNOS activation and oxidative stress in both tissues. MC regulated oxidant/antioxidant had balanced thus preventing heart and aorta damage via eNOS involvement.